KUGR: A Genomics and Bioinformatics Shared Resource
KUGR is available for use by all researchers at UNM and affiliated institutions.
Location: CRF 118 and CRF 124
Shared instruments available Mon-Fri 9:00 am - 4:30 pm
(other times may be available by special arrangement)
Use our iLab site for reservations and quotes.
The Keck-UNM Genomics Resource (KUGR) is a shared resource that provides access to microarrays, real-time PCR and next generation sequencing technologies, coupled with expert bioinformatics analysis. KUGR is available for use by all faculty at UNM and its affiliates, and all investigators are encouraged to contact KUGR to find out how we can help with their research.
- KUGR now offers a variety of next-generation sequencing assays that utilize the Ion Torrent PGM instruments.
- A recent reorganization has formed the new Analytical and Translational Genomics Core (ATGC), which integrates experts in genomics, bioinformatics, biostatistics and biocomputing to produce a new team designed to tackle the analysis of large data sets generated by next-generation sequencing assays.
- The new Ion Torrent Proton whole-genome sequencing instrument should arrive in September. Watch for further details.
List of Services:
The KUGR shared resource has a complete Affymetrix system including two automated hybridization/fluidics stations and a high-resolution GeneChip scanner with autoloader as well as a suite of specialized instruments for the analysis of nucleic acids. The KUGR facility offers a range of services including:
- Affymetrix 3'-Expression Arrays: The standard gene expression arrays use up to 22 probe sets per transcript to measure the abundance of the 3'-ends of up to 45,000 well-characterized and less characterized transcripts.
- Affymetrix SNP Genotyping: By measuring up to 2,000,000 single nucleotide polymorphisms distributed throughout the genome, these arrays are designed for whole genome association studies. The Affymetrix SNP 6.0 arrays can map genetic loci and can detect loss of heterozygosity (LOH) and changes in copy number.
- TaqMan SNP Assays: KUGR uses high throughput real time PCR (TaqMan) assays to measure single nucleotide polymorphisms in human samples. This assay is appropriate for measuring a few or a few dozen SNPs per sample.
- Expert Bioinformatics Data Analysis: KUGR facility staff use sophisticated data analysis methods to analyze gene expression and genotyping data and strive to provide our users with publication-quality figures for their manuscripts or grant applications.
- Real Time PCR: The KUGR Facility staff is highly experienced and can perform real-time PCR assays for UNM laboratories. These assays are ideal for quantitative measurements of gene expression and for detection of human SNPs using commercial assays.
- Epigenetics Assays: Affymetrix promoter tiling arrays have probes centered 35 nt apart across the promoters of 25,000 genes. These arrays are designed for epigenetics assays such as detecting promoter methylation or for performing chromatin immunoprecipitation (ChIP on chip) assays.
- Ion Torrent Next-Generation Sequencing: In collaboration with the laboratory of Dr. Jeremy Edwards, KUGR can perform a variety of next-generation sequencing assays using the Ion Torrent PGM instrument. These include ChIP-seq, RNA-seq and targeted sequencing assays. This platform is also used for Comprehensive Cancer Panel Assays, which performs multiplex targeted sequencing of all exons from over 400 known cancer-relevant genes, starting with very small amounts of material (such as FFPE samples).
Please acknowledge the KUGR facility in your publications !