Molecular Genetics & Microbiology

Dave Peabody

David Peabody, Ph.D.

Molecular Genetics and Microbiology
MSC08 4660
1 University of New Mexico
Albuquerque, NM 87131-0001
 
Office: BRF 337A
Tel: (505) 272-0071
Fax: (505) 272-6029
E-mail: dpeabody@salud.unm.edu

Keywords: Engineering Bacteriophage Particles for Diverse Translational Applications.

Research Interests

Dr. Peabody was trained at Stanford University in the laboratory of Paul Berg (Nobel Prize, 1980) and has been associated with UNM since 1984. For a number of years he studied RNA viruses of bacteria as model systems to understand gene regulation, but in recent years has focused on adapting the virus-like particles of these bacteriophages as platforms for vaccine development and targeted drug delivery.

Selected Publications

Link to PubMed

Epler, K., Padilla, D., Phillips, G., Crowder, P., Castillo, R., Wilkinson, D., Wilkinson, B., Burgard, C., Kalinich, R., Townson, J., Chackerian, B., Willman, C., Peabody, D., Wharton, W., Brinker, C.J., Ashley, C. and E. Carnes (2012).  Delivery of Ricin Toxin A-Chain by Peptide-Targeted Mesoporous silica Nanoparticle-Supported Lipid Bilayers.  Advanced Healthcare Materials Epub 2012 April 2.  DOI: 10.1002/adhm.201200022

Ashley, C.E., Carnes, E.C., Epler, K.E., Padilla, D.P., Phillips, G.K., Castillo, R.E., Wilkinson, D.C., Wilkinson, B.S., Burgard, C.A., Sewell, R.M., Townson, J.L., Chackerian, B., Willman, C.L., Peabody, D.S., Wharton, W., and C.J. Brinker (2012).  Delivery of Small Interfering RNA by Peptide-Targeted Mesoporous Silica Nanoparticle-Supported Lipid Bilayers.  ACS Nano. Epub 2012 February 6. DOI: 10.1021/nn204102q

Tumban, E., Peabody, J., Peabody, D.S., and B. Chackerian (2011). A pan-HPV vaccine based on bacteriophage PP7 VLPs displaying broadly cross-neutralizing epitopes from the HPV minor capsid protein, L2.  PLoS One 2011; 6(8): e23310. Epub 2011 Aug 17.

Ashley CE, Carnes EC, Phillips GK, Durfee PN, Buley MD, Lino CA, Padilla DP, Phillips B, Carter MB, Willman CL, Brinker CJ, Caldeira JD, Chackerian B, Wharton W, and Peabody, DS (2011) Cell-Specific Delivery of Diverse Cargos by Bacteriophage MS2 Virus-like Particles. ACS Nano 2011 Jun 7. [Epub ahead of print].

Caldeira JC and Peabody DS (2011) Thermal stability of RNA phage virus-like particles displaying foreign peptides. J Nanobiotechnology. 2011 May 24;9(1):22.

Chackerian B, Caldeira Jdo C, Peabody J, and Peabody, DS (2011) Peptide epitope identification by affinity selection on Bacteriophage MS2 Virus-Like Particles. J Mol Biol. 2011 Jun 3;409(2):225-37. Epub 2011 Apr 9.

Ashley CE, Carnes EC, Phillips GK, Padilla D, Durfee PN, Brown PA, Hanna TN, Liu J, Phillips B, Carter MB, Carroll NJ, Jiang X, Dunphy DR, Willman CL, Petsev DN, Evans DG, Parikh AN, Chackerian B, Wharton W, Peabody DS, and Brinker CJ (2011)  The targeted delivery of multicomponent cargos to cancer cells by nanoporous particle-supported lipid bilayers. Nat Mater. 2011 May;10(5):389-97. Epub 2011 Apr 17.

Ashley CE, Dunphy DR, Jiang Z, Carnes EC, Yuan Z, Petsev DN, Atanassov PB, Velev OD, Sprung M, Wang J, Peabody DS, and Brinker CJ (2011) Convective Assembly of 2D Lattices of Virus-like Particles Visualized by In-Situ Grazing-Incidence Small-Angle X-Ray Scattering. Small. 2011 Apr 18;7(8):1043-50. Epub 2011 Mar 21.

Caldeira Jdo C, Medford A, Kines RC, Lino CA, Schiller JT, Chackerian B, and Peabody DS (2010)  Immunogenic display of diverse peptides, including a broadly cross-type neutralizing human papillomavirus L2 epitope, on virus-like particles of the RNA bacteriophage PP7. Vaccine. 2010 Jun 17;28(27):4384-93. Epub 2010 Apr 29.

Peabody, DS, Jordan, SK, Caldeira, JC, Manifold-Wheeler, B, Medford, A and Chackerian, B (2007) Immunogenic display of diverse peptides on virus-like particles of RNA phage MS2. J. Mol. Biol. 380: 252-263.

Caldeira, JC and Peabody, DS (2007) Stability and assembly in vitro of bacteriophage PP7 virus-like particles. J. Nanobiotech. 5: 10.

Chackerian, B, Rangel, M, Hunter, Z, and Peabody, DS. (2006) Virus-like particle based immunogens for Alzheimer’s disease induce antibody responses against amyloid-ß without concomitant T cell responses. Vaccine, 24:  6321-6331.

Anderson, EA, Isaacman, S, Peabody, DS, Wang, EY, Canary, JW, and Kershenbaum, K. (2006) Viral nanoparticles donning a paramagnetic coat: conjugation of MRI contrast agents to the MS2 capsid. Nanoletters, 6: 1160-1164.

Lima, S.M.B., Vaz, ACQ, Souza, TLF, Peabody, DS, Silva JL, and Oliveira, AC. (2006) Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability. FEBS J.  273: 1463-1475.

Horn, WT, Tars, K, Grahn, E, Helgstrand, C, Baron, AJ, Lago, H, Adams, CJ, Peabody DS, Phillips, SEV, Stonehouse, NJ, Liljas, L, and Stockley, PG (2006) Structural Basis of RNA Binding Discrimination between Bacteriophages Qß and MS2. Structure 14: 487-495.

Lima, S. M. B., Peabody, D., Silva, J. L., Oliveira, A. C. (2004) Mutations in the hydrophobic core and in the protein-RNA interface affect the packing and pressure stability of MS2 virus capsids. Eur. J. Biochem.271: 135-145.

Peabody, D.S. (2003) A viral platform for chemical modification and multivalent display. J. Nanobiotech. 1: 5.

AnoBom, C.D., Albuquerque, S.C., Albernaz, F.P., Oliveira, A.C., Silva, J.L., Peabody, D.S., Valente, A.P. and Almeida, F.C.L. (2003) Structural Studies of MS2 Bacteriophage Virus Particle Disassembly by Nucloear Magnetic Resonance Relaxation Measurements. Biophys. J. 84: 3894-3903.

Lim, F. and Peabody,D.S. (2002) RNA recognition site of PP7 coat protein. Nucleic Acids Res. 30: 4138-4144.

Spingola, M., Lim, F., and Peabody, D.S. (2002)  Binding of Diverse RNAs by a Single Protein Structural Framework. Archives of Biochemistry and Biophysics 405: 122-129

Peabody, D.S. and Al-Bitar, L. (2001) Isolation of Viral Coat Protein Mutants With Altered Assembly and Aggregation Properties.  Nucleic Acids Research 29: e113.

Lim, F., Downey, T., Peabody D.S. (2001) Specific RNA Binding and Translational Repression by PP7 Coat Protein. J. Biol. Chem. 276: 22507

Powell, A.J., Peabody, D.S. (2001) Asymmetric interactions in the adenosine-binding pockets of the MS2 coat protein dimer. BMC Molecular Biology 2: 6Peabody, D.S. and Chakerian, A. 1999.

Asymmetric Contributions to RNA Binding by the Thr45 Residues of the MS2 Coat Protein Dimer. J. Biol. Chem. 274: 25403-25410.

Convery, M.A., Rowsell, S., Stonehouse, N.J., Ellington, A.D., Hirao, I., Murray, J.B., Peabody, D.S., Phillips, S.E.V., and Stockley, P.G. 1998. Crystal structure of an RNA-aptamer-protein complex at 2.8 angstrom resolution. Nature Structural Biology 5: 133-139.

Rowsell, S., Stonehouse, M.J., Convery, M.A., Adams, C.J., Ellington, A.D., Hirao, I., Peabody, D.S., Stockley, P.G., and Phillips, S.E.V. 1998. Crystal structures of a series of RNA aptamers complexed to the same protein target. Nature Structural Biology 5: 970-975

Spingola, M. and Peabody, D.S. 1997. MS2 coat protein mutants which bind Q-beta RNA. Nucleic Acids Res. 25: 2808-2815.

Peabody, D.S. 1997. Role of the coat protein-RNA interaction in the life cycle of the bacteriophage MS2. Mol. Gen. Genet. 254: 358-364.

Lim, F., Spingola, M., and Peabody, D.S. 1996. The RNA-binding site of Q-beta coat protein. J. Biol. Chem. 271: 31839-31845.

Peabody, D.S. and Lim, F. 1996. Complementation of RNA Binding Site Mutations in Heterodimers of Bacteriophage MS2 Coat Protein. Nucleic Acids Res. 24:2352-2359.

Ni,C.-Z., Syed, R., Kodandapani, R., Wickersham, J., Peabody, D.S., Ely, K.R. 1995. Crystal Structure of th Bacteriophage MS2 Coat Protein Dimer: Implications for Virus Assembly and RNA Binding. Structure 3:255-263.

Lim, F. and Peabody, D.S. 1994. Mutations that Increase the Affinity of a Transitional Repressor for RNA. Nucleic Acids Res. 22:3748-3752.

Lim, F., Spingola, M. and Peabody, D.S. 1994. Altering the RNA Binding Specificity of a Translational Repressor. J. Biol. Chem. 269:9006-9010.

Peabody, D.S. 1993. The RNA-Binding Site of Bacteriophage MS2 Coat Protein. EMBO J. 12:595-600.

Peabody, D.S. and Ely, K.R. 1992. Control of Translational Repression by Protein-Protein Interactions. Nucleic Acids Res. 20:1649-1655.